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American Society of Microbiology ABMM Exam Syllabus Topics:

American Society of Microbiology ABMM Sample Questions:

1. A Gram-positive rod is isolated from the blood culture of a neonate with sepsis. The organism exhibits weak beta-hemolysis on sheep blood agar, is catalase-positive, hydrolyzes esculin in the presence of bile, and demonstrates characteristic "tumbling" motility in a wet mount prepared from a broth culture incubated at 25°C. Which organism is most likely?

A) Erysipelothrix rhusiopathiae
B) Listeria monocytogenes
C) Bacillus anthracis
D) Corynebacterium jeikeium

2. A patient presents with symptoms suggestive of primary syphilis. Which diagnostic test typically becomes positive earliest in the course of infection?

A) Rapid Plasma Reagin (RPR)
B) Venereal Disease Research Laboratory (VDRL) test on CSF
C) Treponema pallidum particle agglutination (TP-PA)
D) Darkfield microscopy of lesion exudate

3. A clinical microbiology laboratory is implementing a new multiplex nucleic acid amplification test (NAAT) for the detection of respiratory viral pathogens. To ensure the quantitative accuracy of the assay, it is essential to include:

A) Internal controls to monitor for PCR inhibition.
B) Only positive and negative controls.
C) External quality assessment samples.
D) Calibration standards with known concentrations of target nucleic acids.

4. Which of the following organisms is an obligate intracellular bacterium transmitted by arthropod vectors and is a common cause of Rocky Mountain Spotted Fever?

A) Coxiella burnetii
B) Chlamydia trachomatis
C) Borrelia burgdorferi
D) Rickettsia rickettsii

5. The azole class of antifungal agents (e.g., fluconazole, voriconazole) exerts its effect primarily by inhibiting which fungal-specific enzyme involved in membrane synthesis?

A) Chitin synthase
B) Beta-(1,3)-glucan synthase
C) Squalene epoxidase
D) Lanosterol 14-alpha-demethylase

Solutions: